sperm penetration assay meaning in English
精子穿透测定
Examples
- In order to investigate the role of mannose receptor ( mr ) of human sperm , the zona free hamster eggs were pre - incubated with purified mr ( pmr ) isolated from motile human sperm by mannose - agarose gel affinity chromatography . the ultrastuctural alteration and cortical granule exocytosis of the eggs were then observed by transmissian electron microscope and tritc - lca immunofluorescence microscope , respectively . the mice were immunized with pmr and the antiserum was raised . after capacitation and induction of the acrosome reaction , the human spermatozoa and oocytes were incubated with the antiserum . then the sperm penetration assay was undertaken
为了进一步探讨人精于mr在精卵融合中的作用，本文采用改良后的甘露糖-琼脂糖凝胶亲和层析法分离纯化人精子mr ，并将提纯的人精子甘露糖受体( purifiedmannosereceptor , pmr )作用于去透明带的金黄地鼠卵母细胞，运用透射电子显微镜技术和罗丹明偶联的兵豆凝集素( tritc - lca )免疫荧光标记技术观察pmr对卵子的影响。 - In order to detect the effect of human sperm mannose - ligand receptor on the fertilization ability , in the study reported here mannose - ligand receptors ( mrs ) were purified from human sperm by modified mannose - agarose gel affinity chromatography coloumn and determined protien concentration by lowry , preincubated zona - free hamster oocytes with four purified mannose - ligand receptor ( pmr ) concentrations before sperm penetration assay ( spa ) to test the pmrs cell biology nature of inhibition to fertilization
本研究用改良后的亲和层析法分离纯化mr ， lowry法测定其蛋白质浓度，在精子穿透试验( spermpenetrationassay ， spa )模型中定量研究其对精卵融合能力的影响并检测其细胞生物学活性；以已知浓度的pmr ( purifiedmannose - ligandreceptor )干预精子半透明带试验，观察用pmr预处理半透明带对精子与透明带结合的影响。